Journal: Biofactors (Oxford, England)

Article Title: Anticancer effect of minor phytocannabinoids in preclinical models of multiple myeloma

doi: 10.1002/biof.2078

Figure Lengend Snippet: CBG, CBC, CBN, and CBDV effect on HuOB cell line viability. HuOB were treated for 72 h and then MTT assay was used to determine cell viability. Three separate experiments are shown as mean ± SD. * p < 0.05, ** p < 0.01, **** p < 0.0001 versus VHC.

Article Snippet: Human osteoblast cell line CI‐huOB (HuOB) was purchased from InSCREENex GmbH (Braunschweig, Germany) and cultured in DMEM glucose high medium (EuroClone, Milan, Italy) supplemented with 100 IU/mL penicillin, 100 mg streptomycin, 10% FBS, 1 mM sodium pyruvate, and 2 mM L‐glutamine.

Techniques: MTT Assay

Journal: Biofactors (Oxford, England)

Article Title: Anticancer effect of minor phytocannabinoids in preclinical models of multiple myeloma

doi: 10.1002/biof.2078

Figure Lengend Snippet: MM cell invading the ECM covered membrane toward HuOB cells, after treatment with CBG, CBC, CBN, and CBDV. (A) Representative image of calcein stained RPMI cells invading the ECM coated membrane. Three random fields were observed under a fluorescence microscope. Magnification 10×. (B) The number of invading cells represent the mean ± SD of three separated experiments. * p < 0.05, ** p < 0.01, **** p < 0.0001 versus UNTREATED.

Article Snippet: Human osteoblast cell line CI‐huOB (HuOB) was purchased from InSCREENex GmbH (Braunschweig, Germany) and cultured in DMEM glucose high medium (EuroClone, Milan, Italy) supplemented with 100 IU/mL penicillin, 100 mg streptomycin, 10% FBS, 1 mM sodium pyruvate, and 2 mM L‐glutamine.

Techniques: Membrane, Staining, Fluorescence, Microscopy